New PDF release: Aging Phenomena: Relationships among Different Levels of

By A. Macieira-Coelho (auth.), Kunio Oota, Masami Iriki, Takashi Makinodan, Lynn S. Baker (eds.)

ISBN-10: 1468437348

ISBN-13: 9781468437348

ISBN-10: 1468437364

ISBN-13: 9781468437362

The challenge of senescence, as mirrored within the background of reli­ gion and philosophy, has lengthy been one of many maximum matters of humankind. by contrast, gerontology as a department of technological know-how remains to be relatively younger. in the past decade, concomitant with fast growth in our realizing of the fundamental existence sciences, giant shops of information approximately organic getting older were amassed. this data, notwithstanding, bobbing up from many clinical disciplines and thinking about various degrees of biologic association, turns out virtually random and covers every little thing from molecules to human societies. Theories complicated to interpret the evidence and to appreciate the mech­ anisms serious about senescence have remained in person, instead of basic, territories. It has lengthy been felt via a few gerontologists that it used to be time for a few of the experts to step again and take a generalist view of gerontology, to think again and reevaluate the end result in their analyt­ ical targets at assorted degrees inside a broader context. a few others might imagine it nonetheless untimely. It appeared, despite the fact that, that most of these who collected in Tokyo at the get together of the XIth foreign Congress of Gerontology have been of the opinion that there has been a lot to be received in trying to find interrelationships one of the proof and theories originated within the diverse degrees of investiga­ tion in an try to discover and delight in the organic drama of senescence as an entity.

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Friedman, Hormonal stimulation of DNA synthesis in primary cultures of adult rat hepatocytes, Proc. Natl. Acad. Sci. USA 13:3519 (1916). 16. A. Ichihara, Relation of the characteristics of liver cells during culture, differentiation, and carcinogenesis, in: "Control Mechanisms in Cancer," W. E. Criss, T. Ono, and J. R. , Raven Press, New York (1916). 11. J. Bausher and W. I. Schaeffer, A diploid rat liver cell culture. I. Characterization and sensitivity to alfatoxin B1, In Vitro 9:286 (1914). 18.

It is, therefore, not unreasonable to assume that the bionsynthesis of the repeating disaccharide units in the chondroitin sulfate chain is affected little by the aging process. DISCUSSION Cartilage proteoglycan is composed of a protein core to which a large number of highly negatively charged chondroitin sulfates and a relatively small number of keratan sulfates are covalently attached, in the model proposed by Heineg£rd and Hascall (21). Recently, Inerot et al. (4) indicated that the structural change of proteoglycan macromolecules of articular cartilage with age was probably due to a diminishing of the chondroitin sulfate-rich region, whereas the hyaluronic acid-binding region and the keratan sulfate-rich region remain comparatively constant.

MATERIALS AND METHODS Lung Cell sytem and Liver Cell System Human lung oells (WI-38 and WI-26) were oultivated for weeks following the oonolusion of their rapid proliferation phase, as desoribed previously (11). These oells will subsequently be referred to as senesoent oells. Epithelial-like oells from fetal human liver were oultivated in a chemioally defined medium supplemented with 10S fetal bovine serum, and in the presenoe of irradiated feeder oells (12). Liver oells were passaged by trypsinization.

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Aging Phenomena: Relationships among Different Levels of Organization by A. Macieira-Coelho (auth.), Kunio Oota, Masami Iriki, Takashi Makinodan, Lynn S. Baker (eds.)


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